CN language
Cytena
B.SIGHT

The B.SIGHT is a microbial isolation platform that uses advanced optical microscopy and CYTENA’s single-cell dispensing technology to enable isolation of the microbial cells. The second generation B.SIGHT revolutionizes microbiology research by enabling rapid and efficient isolation of bacteria from complex microbiome samples or transformed cultures used for strain development.

It consists of a dispenser chip that rapidly produces microdroplets from the sample inside a disposable cartridge, combined with high resolution optical microscopy and real-time image analysis to guarantee the dispensing of single bacteria onto individual wells of a multiwell plate. Microbial product discovery and development projects can now be accelerated using the B.SIGHT. The device can be integrated into automation workflow and provides a cost effective and high-throughput generation of microbial isolates.

 

Key Features & Benefits

 1.Dual-channel high resolution microscopy

Detects even the smallest cells based on size and shape. The system comes with full capability to isolate unlabeled and fluorescently labeled single microorganisms.

 2.High-speed isolation of cells

Accelerate your microorganism workflows with high-speed isolation of single bacteria or yeast into multiwell plates. Process a 96-well plate in 3 minutes and a 384- plate in under 8 minutes.

 3.Assurance of clonality

Nozzle images confirm the clonality of dispensed single prokaryotic cells.

 4.High cell viability

The B.SIGHT improves cell viability thanks to fast, gentle and easy-to-use label-free isolation of single bacteria directly into liquid culture or agar.

 5.Compatible with anaerobic conditions

Small benchtop instrument that integrates into fume hoods and anaerobic chambers. Simply connect the monitor, keyboard and mouse or even use a remote control terminal.

 6.Single-cell genomics of uncultivated bacteria

Dispense single cells from your complex microbiota sample in picolitre droplets directly into the center of the well in 96- or 384-well PCR plates, enabling downstream miniaturization with lysis buffer volumes less than 1 μL.

 7.Automation ready

The B.SIGHT lid opens automatically, making the device compatible with robotic automation workflows. The instrument also has a small footprint and can easily be placed in most biosafety cabinets.

 

Product Applications

1.Synthetic biology

2.Single bacteria isolation

3.Single yeast isolation

4.Microbiome research

5.Anaerobic microorganism isolation

6.Synthetic biology